ABSTRACT
OBJECTIVE:To detect the expressions of five gastric cancer cell metastasis associated genes induced by arsenic trioxide METHODS:The expressions of CD44,P53,nm23,H-ras,PCNA induced by arsenic trioxide were examined by immunohistochemistry method RESULTS:Arsenic trioxide induced the decrease of the expressions of CD44,P53,PCNA in gastric cancer cells;the expressions of nm23 and H-ras were not changed by As2 O 3 CONCLUSION:It is tentatively proved that the antineoplastic action of As2 O 3 may be related to the down-regulation of CD44,P53 and PCNA
ABSTRACT
Objective To test the expression of five cancer metastasis associated genes indu ced by arsenic trioxide. Methods The expression of CD44,p53,nm23 ,H-r as and PCNA induced by arsenic trioxide were examined by immunohistochemistry me thod . Results Arsenic trioxide induced decrease of the expression of C D44,p 53 and PCNA in gastric cancer cells,and the expression of nm23 and H-ras were not changed by As_2O_3. Conclusions Arsenic trioxide inhibits the metastasis of gastric ca ncer cells through reducing the expression of cancer metastasis associated gene s.
ABSTRACT
AIM: To study the effect and mechanism of arsenous oxide (As_2O_3) on pancreatic cancer PC_2 strain. METHODS: The pancreatic cancer PC_2 strain was chosen in this experiment. Apoptosis was detected by TUNEL test. Expression of survivin gene was detected by reverse transcriptase PCR. RESULTS: ① After administration of As_2O_3, the survival number of pancreatic cancer cells decreased significantly in a time-dependent manner (P